Cloned (Comment) | Organism |
---|---|
DNA sequence determination, genomic organization and chromosome localization, functional expression as soluble protein fused to protein A IgG-binding domain in COS-1 cells, secretion into the medium | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | type II membrane protein topology | Homo sapiens | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
95000 | - |
x * 95000, recombinant enzyme, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-alpha-D-galactosamine + chondroitin oligosaccharide | Homo sapiens | involved in chondroitin biosynthesis | UDP + N-acetyl-beta-D-galactosaminyl chondroitin oligosaccharide | - |
? | |
UDP-N-acetyl-alpha-D-galactosamine + chondroitin sulfate oligosaccharide | Homo sapiens | involved in chondroitin sulfate biosynthesis: elongation of growing chondroitin sulfate chain | UDP + N-acetyl-beta-D-galactosaminyl chondroitin sulfate oligosaccharide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q8TDX6 | chondroitin N-acetylgalactosaminyltransferase I, not N-acetylgalactosaminyltransferase I EC 2.4.1.174 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 2 potential N-glycosylation sites, either one or both of which are used in COS-1 cells during expression of the recombinant fusion protein | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bone marrow | - |
Homo sapiens | - |
brain | - |
Homo sapiens | - |
colon | low content | Homo sapiens | - |
heart | - |
Homo sapiens | - |
kidney | - |
Homo sapiens | - |
leukocyte | peripheral blood | Homo sapiens | - |
liver | - |
Homo sapiens | - |
lung | - |
Homo sapiens | - |
peripheral blood | - |
Homo sapiens | - |
placenta | very high content | Homo sapiens | - |
skeletal muscle | - |
Homo sapiens | - |
small intestine | - |
Homo sapiens | - |
spleen | - |
Homo sapiens | - |
thymus | low content | Homo sapiens | - |
thyroid gland | high content | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme also has N-acetylgalactosaminyltransferase I activity, EC 2.4.1.174 | Homo sapiens | ? | - |
? | |
UDP-N-acetyl-alpha-D-galactosamine + chondroitin oligosaccharide | involved in chondroitin biosynthesis | Homo sapiens | UDP + N-acetyl-beta-D-galactosaminyl chondroitin oligosaccharide | - |
? | |
UDP-N-acetyl-alpha-D-galactosamine + chondroitin sulfate oligosaccharide | involved in chondroitin sulfate biosynthesis: elongation of growing chondroitin sulfate chain | Homo sapiens | UDP + N-acetyl-beta-D-galactosaminyl chondroitin sulfate oligosaccharide | - |
? | |
UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide | best acceptor substrate | Homo sapiens | UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide | transfer in beta-1,4-linkage | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 95000, recombinant enzyme, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
chondroitin galactosaminyltransferase | - |
Homo sapiens |
More | the enzyme also has N-acetylgalactosaminyltransferase I activity, EC 2.4.1.174 | Homo sapiens |
More | the human form of this enzyme is a bifunctional glycosyltransferase, which also has the 3-beta-glucuronosyltransferase, EC 2.4.1.226, activity required for the synthesis of the chondroitin sulfate disaccharide repeats. Similar chondroitin synthase co-polymerases can be found in Pasteurella multocida and Escherichia coli. | Homo sapiens |
More | the enzyme shows no 3-beta-glucuronosyltransferase activity, EC 2.4.1.226 | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Homo sapiens |